CovidFranceV1, Main, Exploration, bibRecord, 000849

Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays.

Identifieur interne : 000849 ( Main/Exploration ); précédent : 000848; suivant : 000850

Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays.

Auteurs : Katharine H D. Crawford [États-Unis] ; Rachel Eguia [États-Unis] ; Adam S. Dingens [États-Unis] ; Andrea N. Loes [États-Unis] ; Keara D. Malone [États-Unis] ; Caitlin R. Wolf [États-Unis] ; Helen Y. Chu [États-Unis] ; M Alejandra Tortorici [États-Unis, France] ; David Veesler [États-Unis] ; Michael Murphy [États-Unis] ; Deleah Pettie [États-Unis] ; Neil P. King [États-Unis] ; Alejandro B. Balazs [États-Unis] ; Jesse D. Bloom [États-Unis]

Source :

Viruses [ 1999-4915 ] ; 2020.

RBID : pubmed:32384820

Descripteurs français

KwdFr :
- Anticorps antiviraux (immunologie), Anticorps antiviraux (sang), Anticorps neutralisants (immunologie), Cellules HEK293 (MeSH), Confinement de risques biologiques (MeSH), Glycoprotéine de spicule des coronavirus (analyse), Humains (MeSH), Lentivirus (MeSH), Peptidyl-Dipeptidase A (métabolisme), Plasma sanguin (immunologie), Tests de neutralisation (méthodes).
MESH :
- analyse : Glycoprotéine de spicule des coronavirus.
- immunologie : Anticorps antiviraux, Anticorps neutralisants, Plasma sanguin.
- métabolisme : Peptidyl-Dipeptidase A.
- méthodes : Tests de neutralisation.
- sang : Anticorps antiviraux.
- Cellules HEK293, Confinement de risques biologiques, Humains, Lentivirus.

English descriptors

KwdEn :
- Antibodies, Neutralizing (immunology), Antibodies, Viral (blood), Antibodies, Viral (immunology), Containment of Biohazards (MeSH), HEK293 Cells (MeSH), Humans (MeSH), Lentivirus (MeSH), Neutralization Tests (methods), Peptidyl-Dipeptidase A (metabolism), Plasma (immunology), Spike Glycoprotein, Coronavirus (analysis).
MESH :
- chemical , analysis : Spike Glycoprotein, Coronavirus.
- chemical , blood : Antibodies, Viral.
- chemical , immunology : Antibodies, Neutralizing, Antibodies, Viral.
- chemical , metabolism : Peptidyl-Dipeptidase A.
- immunology : Plasma.
- methods : Neutralization Tests.
- Containment of Biohazards, HEK293 Cells, Humans, Lentivirus.

Abstract

SARS-CoV-2 enters cells using its Spike protein, which is also the main target of neutralizing antibodies. Therefore, assays to measure how antibodies and sera affect Spike-mediated viral infection are important for studying immunity. Because SARS-CoV-2 is a biosafety-level-3 virus, one way to simplify such assays is to pseudotype biosafety-level-2 viral particles with Spike. Such pseudotyping has now been described for single-cycle lentiviral, retroviral, and vesicular stomatitis virus (VSV) particles, but the reagents and protocols are not widely available. Here, we detailed how to effectively pseudotype lentiviral particles with SARS-CoV-2 Spike and infect 293T cells engineered to express the SARS-CoV-2 receptor, ACE2. We also made all the key experimental reagents available in the BEI Resources repository of ATCC and the NIH. Furthermore, we demonstrated how these pseudotyped lentiviral particles could be used to measure the neutralizing activity of human sera or plasma against SARS-CoV-2 in convenient luciferase-based assays, thereby providing a valuable complement to ELISA-based methods that measure antibody binding rather than neutralization.

DOI: 10.3390/v12050513
PubMed: 32384820
PubMed Central: PMC7291041

Affiliations:

Links toward previous steps (curation, corpus...)

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Le document en format XML

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<author><name sortKey="King, Neil P" sort="King, Neil P" uniqKey="King N" first="Neil P" last="King">Neil P. King</name>
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<author><name sortKey="Balazs, Alejandro B" sort="Balazs, Alejandro B" uniqKey="Balazs A" first="Alejandro B" last="Balazs">Alejandro B. Balazs</name>
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<author><name sortKey="Bloom, Jesse D" sort="Bloom, Jesse D" uniqKey="Bloom J" first="Jesse D" last="Bloom">Jesse D. Bloom</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays.</title>
<author><name sortKey="Crawford, Katharine H D" sort="Crawford, Katharine H D" uniqKey="Crawford K" first="Katharine H D" last="Crawford">Katharine H D. Crawford</name>
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<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Division of Basic Sciences and Computational Biology Program, Fred Hutchinson Cancer Research Center, Seattle, WA 98109</wicri:regionArea>
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<affiliation wicri:level="4"><nlm:affiliation>Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Genome Sciences, University of Washington, Seattle, WA 98195</wicri:regionArea>
<placeName><region type="state">Washington (État)</region>
<settlement type="city">Seattle</settlement>
</placeName>
<orgName type="university">Université de Washington</orgName>
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<affiliation wicri:level="4"><nlm:affiliation>Medical Scientist Training Program, University of Washington, Seattle, WA 98195, USA.</nlm:affiliation>
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<author><name sortKey="Eguia, Rachel" sort="Eguia, Rachel" uniqKey="Eguia R" first="Rachel" last="Eguia">Rachel Eguia</name>
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</placeName>
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<author><name sortKey="Dingens, Adam S" sort="Dingens, Adam S" uniqKey="Dingens A" first="Adam S" last="Dingens">Adam S. Dingens</name>
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</placeName>
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<author><name sortKey="Loes, Andrea N" sort="Loes, Andrea N" uniqKey="Loes A" first="Andrea N" last="Loes">Andrea N. Loes</name>
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<author><name sortKey="Malone, Keara D" sort="Malone, Keara D" uniqKey="Malone K" first="Keara D" last="Malone">Keara D. Malone</name>
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<author><name sortKey="Wolf, Caitlin R" sort="Wolf, Caitlin R" uniqKey="Wolf C" first="Caitlin R" last="Wolf">Caitlin R. Wolf</name>
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<author><name sortKey="Chu, Helen Y" sort="Chu, Helen Y" uniqKey="Chu H" first="Helen Y" last="Chu">Helen Y. Chu</name>
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<author><name sortKey="Tortorici, M Alejandra" sort="Tortorici, M Alejandra" uniqKey="Tortorici M" first="M Alejandra" last="Tortorici">M Alejandra Tortorici</name>
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<affiliation wicri:level="3"><nlm:affiliation>Institute Pasteur & CNRS UMR 3569, Unité de Virologie Structurale, 75015 Paris, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
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<author><name sortKey="Veesler, David" sort="Veesler, David" uniqKey="Veesler D" first="David" last="Veesler">David Veesler</name>
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<settlement type="city">Seattle</settlement>
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<author><name sortKey="Murphy, Michael" sort="Murphy, Michael" uniqKey="Murphy M" first="Michael" last="Murphy">Michael Murphy</name>
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<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Institute for Protein Design, University of Washington, Seattle, WA 98195</wicri:regionArea>
<placeName><region type="state">Washington (État)</region>
<settlement type="city">Seattle</settlement>
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<orgName type="university">Université de Washington</orgName>
</affiliation>
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<author><name sortKey="Pettie, Deleah" sort="Pettie, Deleah" uniqKey="Pettie D" first="Deleah" last="Pettie">Deleah Pettie</name>
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<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Institute for Protein Design, University of Washington, Seattle, WA 98195</wicri:regionArea>
<placeName><region type="state">Washington (État)</region>
<settlement type="city">Seattle</settlement>
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<author><name sortKey="King, Neil P" sort="King, Neil P" uniqKey="King N" first="Neil P" last="King">Neil P. King</name>
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<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Biochemistry, University of Washington, Seattle, WA 98109</wicri:regionArea>
<placeName><region type="state">Washington (État)</region>
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<author><name sortKey="Bloom, Jesse D" sort="Bloom, Jesse D" uniqKey="Bloom J" first="Jesse D" last="Bloom">Jesse D. Bloom</name>
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<series><title level="j">Viruses</title>
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<imprint><date when="2020" type="published">2020</date>
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<term>Antibodies, Viral (blood)</term>
<term>Antibodies, Viral (immunology)</term>
<term>Containment of Biohazards (MeSH)</term>
<term>HEK293 Cells (MeSH)</term>
<term>Humans (MeSH)</term>
<term>Lentivirus (MeSH)</term>
<term>Neutralization Tests (methods)</term>
<term>Peptidyl-Dipeptidase A (metabolism)</term>
<term>Plasma (immunology)</term>
<term>Spike Glycoprotein, Coronavirus (analysis)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Anticorps antiviraux (immunologie)</term>
<term>Anticorps antiviraux (sang)</term>
<term>Anticorps neutralisants (immunologie)</term>
<term>Cellules HEK293 (MeSH)</term>
<term>Confinement de risques biologiques (MeSH)</term>
<term>Glycoprotéine de spicule des coronavirus (analyse)</term>
<term>Humains (MeSH)</term>
<term>Lentivirus (MeSH)</term>
<term>Peptidyl-Dipeptidase A (métabolisme)</term>
<term>Plasma sanguin (immunologie)</term>
<term>Tests de neutralisation (méthodes)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Spike Glycoprotein, Coronavirus</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Neutralizing</term>
<term>Antibodies, Viral</term>
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</keywords>
<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Glycoprotéine de spicule des coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term>
<term>Anticorps neutralisants</term>
<term>Plasma sanguin</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Plasma</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Neutralization Tests</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Peptidyl-Dipeptidase A</term>
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<keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Tests de neutralisation</term>
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<keywords scheme="MESH" xml:lang="en"><term>Containment of Biohazards</term>
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<term>Humans</term>
<term>Lentivirus</term>
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<keywords scheme="MESH" xml:lang="fr"><term>Cellules HEK293</term>
<term>Confinement de risques biologiques</term>
<term>Humains</term>
<term>Lentivirus</term>
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<front><div type="abstract" xml:lang="en">SARS-CoV-2 enters cells using its Spike protein, which is also the main target of neutralizing antibodies. Therefore, assays to measure how antibodies and sera affect Spike-mediated viral infection are important for studying immunity. Because SARS-CoV-2 is a biosafety-level-3 virus, one way to simplify such assays is to pseudotype biosafety-level-2 viral particles with Spike. Such pseudotyping has now been described for single-cycle lentiviral, retroviral, and vesicular stomatitis virus (VSV) particles, but the reagents and protocols are not widely available. Here, we detailed how to effectively pseudotype lentiviral particles with SARS-CoV-2 Spike and infect 293T cells engineered to express the SARS-CoV-2 receptor, ACE2. We also made all the key experimental reagents available in the BEI Resources repository of ATCC and the NIH. Furthermore, we demonstrated how these pseudotyped lentiviral particles could be used to measure the neutralizing activity of human sera or plasma against SARS-CoV-2 in convenient luciferase-based assays, thereby providing a valuable complement to ELISA-based methods that measure antibody binding rather than neutralization.</div>
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<DateCompleted><Year>2020</Year>
<Month>05</Month>
<Day>12</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>08</Month>
<Day>28</Day>
</DateRevised>
<Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1999-4915</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>12</Volume>
<Issue>5</Issue>
<PubDate><Year>2020</Year>
<Month>05</Month>
<Day>06</Day>
</PubDate>
</JournalIssue>
<Title>Viruses</Title>
<ISOAbbreviation>Viruses</ISOAbbreviation>
</Journal>
<ArticleTitle>Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays.</ArticleTitle>
<ELocationID EIdType="pii" ValidYN="Y">E513</ELocationID>
<ELocationID EIdType="doi" ValidYN="Y">10.3390/v12050513</ELocationID>
<Abstract><AbstractText>SARS-CoV-2 enters cells using its Spike protein, which is also the main target of neutralizing antibodies. Therefore, assays to measure how antibodies and sera affect Spike-mediated viral infection are important for studying immunity. Because SARS-CoV-2 is a biosafety-level-3 virus, one way to simplify such assays is to pseudotype biosafety-level-2 viral particles with Spike. Such pseudotyping has now been described for single-cycle lentiviral, retroviral, and vesicular stomatitis virus (VSV) particles, but the reagents and protocols are not widely available. Here, we detailed how to effectively pseudotype lentiviral particles with SARS-CoV-2 Spike and infect 293T cells engineered to express the SARS-CoV-2 receptor, ACE2. We also made all the key experimental reagents available in the BEI Resources repository of ATCC and the NIH. Furthermore, we demonstrated how these pseudotyped lentiviral particles could be used to measure the neutralizing activity of human sera or plasma against SARS-CoV-2 in convenient luciferase-based assays, thereby providing a valuable complement to ELISA-based methods that measure antibody binding rather than neutralization.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Crawford</LastName>
<ForeName>Katharine H D</ForeName>
<Initials>KHD</Initials>
<AffiliationInfo><Affiliation>Division of Basic Sciences and Computational Biology Program, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Medical Scientist Training Program, University of Washington, Seattle, WA 98195, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Eguia</LastName>
<ForeName>Rachel</ForeName>
<Initials>R</Initials>
<AffiliationInfo><Affiliation>Division of Basic Sciences and Computational Biology Program, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Dingens</LastName>
<ForeName>Adam S</ForeName>
<Initials>AS</Initials>
<AffiliationInfo><Affiliation>Division of Basic Sciences and Computational Biology Program, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Loes</LastName>
<ForeName>Andrea N</ForeName>
<Initials>AN</Initials>
<AffiliationInfo><Affiliation>Division of Basic Sciences and Computational Biology Program, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Malone</LastName>
<ForeName>Keara D</ForeName>
<Initials>KD</Initials>
<AffiliationInfo><Affiliation>Division of Basic Sciences and Computational Biology Program, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wolf</LastName>
<ForeName>Caitlin R</ForeName>
<Initials>CR</Initials>
<AffiliationInfo><Affiliation>Division of Allergy and Infectious Diseases, University of Washington, Seattle, WA 98195, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Chu</LastName>
<ForeName>Helen Y</ForeName>
<Initials>HY</Initials>
<AffiliationInfo><Affiliation>Division of Allergy and Infectious Diseases, University of Washington, Seattle, WA 98195, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Tortorici</LastName>
<ForeName>M Alejandra</ForeName>
<Initials>MA</Initials>
<AffiliationInfo><Affiliation>Department of Biochemistry, University of Washington, Seattle, WA 98109, USA.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Institute Pasteur & CNRS UMR 3569, Unité de Virologie Structurale, 75015 Paris, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Veesler</LastName>
<ForeName>David</ForeName>
<Initials>D</Initials>
<AffiliationInfo><Affiliation>Department of Biochemistry, University of Washington, Seattle, WA 98109, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Murphy</LastName>
<ForeName>Michael</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Pettie</LastName>
<ForeName>Deleah</ForeName>
<Initials>D</Initials>
<AffiliationInfo><Affiliation>Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>King</LastName>
<ForeName>Neil P</ForeName>
<Initials>NP</Initials>
<AffiliationInfo><Affiliation>Department of Biochemistry, University of Washington, Seattle, WA 98109, USA.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Balazs</LastName>
<ForeName>Alejandro B</ForeName>
<Initials>AB</Initials>
<AffiliationInfo><Affiliation>The Ragon Institute of Massachusetts General Hospital, the Massachusetts Institute Technology, and Harvard University, Cambridge, MA 02139, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Bloom</LastName>
<ForeName>Jesse D</ForeName>
<Initials>JD</Initials>
<AffiliationInfo><Affiliation>Division of Basic Sciences and Computational Biology Program, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Howard Hughes Medical Institute, Seattle, WA 98103, USA.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<GrantList CompleteYN="Y"><Grant><GrantID>DP2DA040254</GrantID>
<Acronym>DA</Acronym>
<Agency>NIDA NIH HHS</Agency>
<Country>United States</Country>
</Grant>
<Grant><GrantID>R01GM120553</GrantID>
<Acronym>GM</Acronym>
<Agency>NIGMS NIH HHS</Agency>
<Country>United States</Country>
</Grant>
<Grant><GrantID>Pew Biomedical Scholars Award</GrantID>
<Agency>Pew Charitable Trusts</Agency>
<Country>International</Country>
</Grant>
<Grant><GrantID>R01 AI141707</GrantID>
<Acronym>AI</Acronym>
<Agency>NIAID NIH HHS</Agency>
<Country>United States</Country>
</Grant>
<Grant><GrantID>Investigator Research Budget</GrantID>
<Acronym>HHMI</Acronym>
<Agency>Howard Hughes Medical Institute</Agency>
<Country>United States</Country>
</Grant>
<Grant><GrantID>F30AI149928</GrantID>
<Agency>National Institute of Allergy and Infectious Diseases</Agency>
<Country>International</Country>
</Grant>
<Grant><GrantID>HHSN272201700059C</GrantID>
<Acronym>AI</Acronym>
<Agency>NIAID NIH HHS</Agency>
<Country>United States</Country>
</Grant>
<Grant><GrantID>Investigators in the Pathogenesis of Infectious Disease Award</GrantID>
<Agency>Burroughs Wellcome Fund</Agency>
<Country>International</Country>
</Grant>
<Grant><GrantID>R01AI141707</GrantID>
<Agency>National Institute of Allergy and Infectious Diseases</Agency>
<Country>International</Country>
</Grant>
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<PublicationType UI="D052060">Research Support, N.I.H., Intramural</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2020</Year>
<Month>05</Month>
<Day>06</Day>
</ArticleDate>
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<MedlineJournalInfo><Country>Switzerland</Country>
<MedlineTA>Viruses</MedlineTA>
<NlmUniqueID>101509722</NlmUniqueID>
<ISSNLinking>1999-4915</ISSNLinking>
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<NameOfSubstance UI="D064370">Spike Glycoprotein, Coronavirus</NameOfSubstance>
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<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
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<MeshHeading><DescriptorName UI="D016086" MajorTopicYN="N">Lentivirus</DescriptorName>
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<QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName>
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<MeshHeading><DescriptorName UI="D007703" MajorTopicYN="N">Peptidyl-Dipeptidase A</DescriptorName>
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<MeshHeading><DescriptorName UI="D010949" MajorTopicYN="N">Plasma</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D064370" MajorTopicYN="N">Spike Glycoprotein, Coronavirus</DescriptorName>
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<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">293T-ACE2</Keyword>
<Keyword MajorTopicYN="Y">ACE2</Keyword>
<Keyword MajorTopicYN="Y">ALAYT</Keyword>
<Keyword MajorTopicYN="Y">COVID-19</Keyword>
<Keyword MajorTopicYN="Y">SARS-CoV-2</Keyword>
<Keyword MajorTopicYN="Y">Spike</Keyword>
<Keyword MajorTopicYN="Y">coronavirus</Keyword>
<Keyword MajorTopicYN="Y">cytoplasmic tail</Keyword>
<Keyword MajorTopicYN="Y">lentiviral pseudotype</Keyword>
<Keyword MajorTopicYN="Y">luciferase</Keyword>
<Keyword MajorTopicYN="Y">neutralization assay</Keyword>
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<CoiStatement>H.Y.C. is a consultant for Merck and Glaxo Smith Kline and receives research funding from Sanofi Pasteur. The other authors declare no conflicts of interest.</CoiStatement>
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Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays.

Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays.

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